HSP90 complex antibody from Biorbyt

Application Notes: Goat anti-mouse IgM was used to bind 25 µl of protein G-Sepharose. SMC-109 IgM from 0.5 ml of high speed supernatant medium was loaded onto the IgG resin and incubated with 100 µl of rabbit reticulocyte lysate for 30 min. at 30C. After washing (4X1 ml), bound proteins were resolved on SDS PAGE, including HSP90, HSP70 and Hop.

Application Dilutions: ICC/IF (1:100), IP (1:1000); optimal dilutions for assays should be determined by the user.